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Nonetheless, the results of later experiments refuted these theories and confirmed that throughout the acidity-alkalinity range encountered in natural waters the pH is of little importance in nitrite toxicity. The effect of chloride on nitrite toxicity is so marked that the outcomes of exams made with out recording the chloride concentrations in the water cannot be in contrast with these of other tests. On this investigation, the 96h LC50 for rainbow trout ranged from 0.24 to 12.20 mg per litre, depending on the chloride content material of the dilution water (in this case the chloride content material ranged from zero.35 to forty.9 mg per litre).

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